US20110165626A1 - High Yield Production of Sialic Acid (Neu5ac) by Fermentation - Google Patents
191.00 £
Published date: 2024/08/03
- Location: 12040, Town of Clear Creek, Michigan, United States
In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 mm NaCl, 2 mm tris(2-carboxyethyl)phosphine, pH 7.5), and finally concentrated to 0.5-1 mm for storage at 4 °C. If you have any sort of questions regarding where and the best ways to utilize website, you could contact us at our web site. All 1D NMR experiments were performed using a Bruker Advance I 500-MHz spectrometer with a 5-mm PATXI 1H/D-13C/15N Z-GRD probe at 293 K.
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